Modern techniques of Raman spectroscopy:
mapping and optical activity (ROA)

The seminar will focus on two modern spectroscopic techniques: Raman mapping and Raman Optical Activity spectroscopy (ROA). In the lecture part fundamentals of both will be presented. The accompanying seminar workshop will include presentation of the equipment and its measurement capabilities, particularly for studies of biological material.

20-22 June 2012, Cracow

June 20, 2012 (Wednesday)

• 15.00 Presentation of the Bruker company representative
   Dariusz Marek i Michael Huber
Methods complementary to Raman spectroscopy: IR imaging - lectures and presentation of equipment and software
• 17.00-18.00 Communications (Dr Kamilla Malek, MSc Tomasz Wróbel)
• 18.15 - Get-togheter with wine tasting

LUMOS FT-IR Microscope LUMOS is a fully automated stand-alone FT-IR microscope. It combines best performance for visual inspection and infrared spectral analysis of micro samples with highest comfort in use.

June 21, 2012 (Thursday)
Raman Mapping
• 9.00 – 11.00 Presentation of the Witec company's representatives
   Dr. Jan Toporski "Introduction to Witec and Witec microscopes"
   Dr. Maxime Tchaya "Instrumentation and application of Confocal Raman imaging combination to other analysis methods (AFM-SNOM-Optical Profilometry)"
   Dr. Andreas Bergner - LOT-ORIEL, Niemcy "Nanoscale Biomaterial Deposition & IR mapping with nm resolution"
• 11.00 - 11.30 Coffee break
• 11.30 – 13.00 Communications (Dr Agnieszka Kaczor, Dr Katarzyna Marzec, Dr Anna Ryguła, MSc Katarzyna Majzner, MSc Emilia Staniszewska)
• 13.00 – 14.00 Lunch Break
• 14.00 – 18.00 Presentation of the equipments in groups / training (presentation of devices and software: Raman Mapping, AFM, SNOM, fluorescence microscopy, nano-lithography, femtosecond systems)
• 18.15 – Beer evening with surprise

The Raman Alpha 300 with its unique design allows the measurement of confocal microscopy, SNOM and AFM. Confocal Raman Imaging Option Upgrade for SNOM allows the simultaneous recording of Raman images and obtaining information about the sample surface topography using the AFM technique. The use of the microscope rotating turret allows the use of selected techniques (Raman, SNOM or AFM) without moving the sample.
June 22, 2012 (Friday)
Raman optical activity
• 9.00 – 11.00 Presentation of the representative of the Bio-Tools - Frank Trundle
   Dr Christian Johannessen Post Doctoral Research Associate, Manchester Interdisciplinary Biocentre "Employing Raman optical activity in conformational analysis: From small organics to proteins"
   Dr Shi Qui Postdoctoral researcher, Antwerp University Belgium "ROA studies on the pH- Dependent Chirality of L-proline in aqueous solution"
• 11.00 - 11.30 Coffee break
• 11.30 – 13.30 Presentation of the equipments in groups / training
• 13.30 – 14.30 Lunch Break

Raman Optical Activity (ROA)
ChiralRAMAN BioTools Raman Optical Activity (ROA) spectrometer
ROA (Raman Optical Activity) is a method complementary to vibrational circular dichroism (VCD).
• from 14.30 – Individual consultations, measurements of samples

• Place: Hall of Athens, Jagiellonian Centre for Experimental Therapeutics (JCET), 14 Bobrzyńskiego St, Cracow
• Fee: until 10 June 2012 - 200 PLN per person (including the practical and theoretical training, office and conference materials, coffee breaks, lunch, dinners).

Jagiellonian Centre for Experimental Therapeutics
14 Bobrzyńskiego St, Crakow
Account No.
87 1240 4722 1111 0000 4854 4672
transfer title: "seminar_name"

• Assoc. Małgorzata Barańska
• Dr. Katarzyna Chruszcz-Lipska
• Dr. Krzysztof Gębski (Secretary, tel. 12-6632064,
• MSc Aleksandra Jaworska
• MSc Tomasz Wróbel