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Biocrystallography

 

 

group members

 
Justyna Kalinowska-Tłuścik, Dr habil, PhD
 
Katarzyna Kurpiewska, PhD     - www
 
Joanna Lipowska, PhD, Eng
 
Joanna Loch, PhD     - www
 
Wojciech Nitek, PhD
 
Monika Siuda, Msc
 
Paulina Wróbel, Msc

basic information

 

 



Main fields of research activity

  1. Crystal structure investigations of selected proteins
  2. High pressure crystal structure and structural stability of proteins
  3. Mechanism of enzymatic activity and its inhibition



Research grants

Beta-lactoglobulin as a potential carrier of drugs grant N204 324037, 2009 - 2011

Intermediate state conformations in high pressure protein structures grant N204 125 32/3170, 2007 - 2010

Structural investigations of conformationl stability of RNaze A variants grant 1 T09A 108 30 , 2006 - 2007

High Pressure Tuning of Biochemical Processes: Protein folding and molecular diseases COST - European Cooperation in the field of Scientific and Technical Research, akcja D30, 2003-2007

Selected Papers
  1. Koziel, M., Podgajny, R., Kania, R., Lebris, R., Mathoniere, C., Lewinski, K., Kruczala, K., Rams, M., Labrugere, C., Bousseksou, A., Sieklucka, B. "Series of MI[Co(bpy)3][Mo(CN)8]·nH2O (MI = Li (1), K (2), Rb (3), Cs (4); n = 7-8) Exhibiting Reversible Diamagnetic to Paramagnetic Transition Coupled with Dehydration-Rehydration Process" Inorganic Chem. (2010) 49, 2765-2772
  2. B. Stopa, B. Piekarska, L. Konieczny, M. Król, J. Rybarska, A. Jagusiak, P. Spólnik, I. Roterman, B. Urbanowicz, P. Piwowar, K. Lewiński "Formation of amyloid-like aggregates through the attachment of protein molecules to a Congo red scaffoding framework ordered under the influence of an electric field" Centr. Eur. J. Chem. (2010) 8, 41-50
  3. D. Matoga, J. Szklarzewicz, K. Lewiński "Novel transformation of molybdenum-coordinated 2-pyrazinecarbonitryle ligand: Nucleophilic addition of cyanide to carbon-nitrogen triple bond" Polyhedron (2010) 29, 94-99
  4. A. Bouet, M. Cieslikiewicz, K. Lewiński, G. Couderat, I. Gillaizeau "Acyclic ene-carbamate. A useful tool for an original synthesis of phosphine-containing alpha-amino acids bearing a quaternary carbon" Tetrahedron (2010) 66, 498-503
  5. K. Kurpiewska, J. Font, M. Ribo, M. Vilanova, K. Lewinski "X-ray crystallographic studies of RNase A variants engineered at the most destabilizing positions of the main hydrophobic core: further insight into protein stability" Proteins: Structure, Function and Bioinformatics (2009) 77, 658-669
  6. W. Przybylski, R. Gryboś, D. Rehder, M. Ebel, W. Łasocha, M. Grzywa, K. Lewiński, J. T. Szklarzewicz "Role of alkali metal ion and hydrogen bonds in M[VO(O2)2bpy]•nH2O•mH2O2 (M = Li+–Cs+) complexes. Part I: The crystal structures and spectroscopic properties" Polyhedron (2009) 28, 1429-1436
  7. V. Chagnault, P. Compain, K. Lewinski, K. Ikeda, N. Asano, O. R. Martin "Stereodivergent Access to Polyhydroxylated 10-Azabicyclo[4.3.1]decanes as New Calystegine Analogs" J. Org. Chem. (2009) 74 (8), 3179-3182
  8. D. Matoga, J. Szklarzewicz, K. Lewinski "Cyanooxo molybdenum(IV) complexes with 2-pyrazinecarbonitrile and 2-pyrazinecarboxylate: Crystallographic and spectroscopic studies reveal metal-assisted nitrile hydrolysis" Polyhedron (2008) 27, 2643-2649
  9. P. Przychodzeń, R. Pełka, K. Lewiński, J. Supel, M. Rams, K. Tomala, B. Sieklucka "Tuning of Magnetic Properties of Polynuclear Lanthanide(III)-Octacyanotungstate(V) Systems: Determination of Ligand-Field Parameters and Exchange Interaction" Inorg. Chemistry (2007) 46, 8924-8938
  10. J. Szklarzewicz, D. Matoga, K. Lewinski "Photoinduced decomposition of hydrazine in K4[Mo(CN)8] solution. X-ray crystal structure of (PPh4)2[Mo(CN)4O(NH3)].2H2O" Inorg. Chim. Acta (2007) 360, 2002-2008
  11. T. Stawski, J. Szklarzewicz, K. Lewinski "The transition products in substitutional photochemistry of M(NC)84- theoretical approach. X-ray crystal structure of (AsPh4)[W(bpy)(CN)6)]" Transition Metal Chemistry (2006) 31, 353-361
  12. P. Przychodzeń, K. Lewiński, M. Bałanda, R. Pełka, K. Tomala, B. Sieklucka "[Ln(terpy)]3+ (Ln = Sm, Gd) Entity Forms Isolated Magnetic Chain with [W(CN)8]3-" Dalton Transactions (2006), 4, 625-628
  13. K. Lewinski, J. Eilmes "Weak Intermolecular Interactions in the (1:1) Pyridine Solvate of Dibenzotetraaza[14]annulene Copper(II) Complex. Crystal Structure of [7,16-dibenzoyl-6,8,15,17-tetramethyl-5,14-dihydrodibenzo[b,i][1,4,8,11] tetraazacyclotetradecinato(2-)-k4 N]copper(II)•pyridine" J. Inclusion Phenomena and Macrocyclic Chemistry (2005) 52, 261-266
  14. P. Przychodzeń, K. Lewiński, M. Bałanda, R. Pełka, M. Rams, T. Wasiutyński, C. Guyard-Duhayon, M. Verdaguer, B. Sieklucka "Crystal Structures and Magnetic Properties of Two Low-Dimensional Materials Constructed From [MnIII(salen)H2O]+ and [M(CN)8]3-/4- (M=Mo, W) Precursors" Inorg. Chem (2004) 43, 2967-2974
  15. T. Korzeniak, R. Podgajny, N. W. Alcock, K. Lewiński, M. Bałanda, T. Wasiutyński, B. Sieklucka "A new family of magnetic 2D coordination polymers based on [Mv(CN)8]3- (M=Mo, W) and pre-programmed Cu2+ centres" Polyhedron (2003) 22, 2183-2190
  16. K. Chruszcz, M. Barańska, K. Lewinski, L. M. Proniewicz "Single Crystal Structure and Vibrational Study of Pyridinephosphonocarboxylic Acid" Vibrational Spectroscopy (2003) 32, 199-206
  17. M. Ciechanowicz-Rutkowska, K. Lewinski, B. Oleksyn, B. Stec: "Model Studies of the function of blockers on the small conductance potassium ion channel" J. Peptide Res. 62, 1-9 (2003)
  18. M. Chruszcz, P. Laidler, M. Monkiewicz, E. Ortlund, L. Lebioda, K. Lewinski: "Crystal structure of a covalent intermediate of endogenous human arylsulfatase A" Journal of Inorganic Biochemistry 96, 386-392 (2003)
  19. K. Lewinski, M. Chruszcz, D. Ksiazek, P. Laidler: "Crystallization and preliminary crystallographic analysis of a new crystal form of arylsulfatase A isolated from human placenta" Acta Cryst. D56, 650-652 (2000)
  20. E. Ortlund, M. W. LaCount, K. Lewinski, L. Lebioda: "Reactions of Pseudomonas 7A Glutaminase-Asparaginase with Diazo Analogs of Glutamine and Asparagine Result in Unexpected Covalent Inhibitions and Suggests an Unusual Catalytic Triad Thr-Tyr-Glu" Biochemistry 39, 1199-1204 (2000)
  21. C. G. Jakob, K. Lewinski, R. Kuciel, W. Ostrowski, L. Lebioda: "Crystal Structure of Prostatic Acid Phosphatase" Prostate 42, 211-218 (2000)
  22. E. Ilkowska, K. Lewinski, R. van Eldik, G. Stochel: "Kinetic, structural and electrostatic aspects of the reduction of pentacyanoferrate(III) complexes by myoglobin" J. Biol. Inorg. Chem. 4, 302-310 (1999)
  23. L. Lovelace, K. Lewinski, C. G. Jakob, R. Kuciel, W. S. Ostrowski, L. Lebioda: "Prostatic Acid Phosphatase: Structural Aspects of Inhibition by L-(+)-Tartrate Ions" Acta Bioch. Polonica 44, 673-678 (1997)
  24. Jakob, C.G., Lewinski, K., LaCount, M.W., Roberts, J. & Lebioda, L.: "Ion Binding Induces Closed Conformation in Pseudomonas 7A Glutaminase-asparaginase (PGA): Crystal Structure of the PGA-SO42--NH4+ Complex at 1.7A Resolution." Biochemistry 36, 923-931 (1997)

Selected conference presentations
  1. J. Loch, K. Kurpiewska, K. Lewiński "Crystal structure of beta-lactoglobuline complexes with biologically important compounds" XX International School of Physics and Chemistry of Condensed Matter, Białowieża 4-11 lipca 2009
  2. K.Lewiński, K. Kurpiewska "High pressure protein crystallography" XX International School of Physics and Chemistry of Condensed Matter, Białowieża 4-11 lipca 2009
  3. K.Lewiński, K. Kurpiewska, K. Dziubek, A. Katrusiak, J. Font, M. Ribo, M. Vilanova "Influence of high pressure on crystal structure of bovine pancreratic ribonuclease" Eighteen Croatian-Slovenian Crystallographic Meeting, Varazdin, Croatia, 17-21 czerwca 2009
  4. K. Lewiński, K. Kurpiewska "High-pressure crystallography of proteins" Frolic Goats High-Pressure Diffraction Workshop, Poznań 19-22 kwietna 2009
  5. Cieslikiewicz, M., Lewinski, K., Coudert, G., Gillaizeau, I. "Préparation et étude structurale de nouveaux composés organophosphorés hétérocycliques" Journée Régionale Centre Ouest de la Société Française de Chimie Francja, Limoges, 11 listopad 2008
  6. J. Loch, M. Myśliwy, K. Lewiński "High pressure crystal structure of insulin - Pressure as a tool to study protein folding" 4th Central European Conference Chemistry towards Biology Węgry, Dobogókő, 8-11 września 2008
  7. M. Sikora, M. Pasenkiewicz-Gierula, K. Lewiński "Molecular dynamics simulation of RNase A at high pressure" 10ta Międzynarodowa konferencja Bioinformatics 2008 Warszawa, 24 - 27 kwietnia 2008
  8. K. Lewiński "Protein under pressure - new approach to protein conformational studies" XXXV Szkoła Zimowa Wydziału Biochemii, Biofizyki i Biotechnologii UJ, Zakopane 23 - 27 lutego 2008
  9. K. Lewiński, K. Kurpiewska, K. Dziubek, A. Katrusiak, J. Font, M. Ribo, M. Vilanova "High pressure crystal structure of ribonuclease A" COST Action D30 Final Group Meeting, Bordeaux, Francja, 25-29 X 2007
  10. I.Stawoska, M.Brindell, J. Supel, A. Skoczowski, K. Lewinski, R. van Eldik and G. Stochel, "Adducts of anticancer ruthenium(III) complexes with human serum albumin (HSA). Influence of HSA on the redox properties of Ru(III) complexes" 2nd European Conference on Chemistry for Life Sciences, 4-8.IX.2007,Wrocław
  11. K. Lewiński, K. Kurpiewska, J. Font, M. Ribó, M. Vilanova "Crystallographic studies on structural stability of RNase A variants" Sixteenth Croatian-Slovenian Crystallographic Meeting, Zadar, Chorwacja, 13?17.06.2007
  12. K. Lewiński, K. Kurpiewska "Progress in crystallographic investigation of high pressure structure of RNase A" COST Action D30 Group Meeting, Hiszpania, Girona, 1 XII 2006
  13. I. Stawoska, J. Supel, A. Wąsik, A. Skoczowski, G. Stochel, K. Lewiński "Interaction of antitumor ruthenium(III) complex, Him trans-[RuCl4(DMSO)(Im)] with human serum albumin" V Symposium of Medical Physics, Ustroń, 20-23.09.2006
  14. J. Supel, P. Hińcza-Stępień, B. Oleksyn, K. Lewiński "Crystallographic investigations of interactions between hemoglobin and antimalarial drugs" International School of Crystallography, 38th Course: The Structure and Function of Large Molecular Assemblies, Erice, Włochy , 9-18.06.2006
  15. M. Bielak, G. Chai, K. Lewinski, L. Lebioda "Structural studies of allosteric inhibiton of N10formyltetrahydrofolate synthetase" 3rd Central European Conference Chemistry Towards Biology, Kraków, 8-13 września 2006
  16. K. Lewinski, M. Bielak, P. Laidler "Kinetic and structural studies of inhibition of Arylsulfatase A" 3rd Central European Conference Chemistry Towards Biology, Kraków, 8-13 września 2006
  17. M. Bielak, G. Chai, K. Lewinski, L. Lebioda "Inhibitory Complexes of N10-formyltetrahydrofolate Synthetase Indicate Negative Cooperativity between Subunits" 23th European Crystallographic Meeting, Belgium, Leuven, 6-11 August 2006
  18. K.Kurpiewska, J. Font, M. Ribó, M. Vilanova, K. Lewiński "Structural insight into protein stability - bovine pancreatic ribonuclease A variants studies" 23th European Crystallographic Meeting, Belgium, Leuven, 6-11 August 2006
  19. I. Stawoska, S. Gawęda, P. Laidler, K. Lewiński, G. Stochel "Kinetic studies on the hydrolysis of sulfate-ester bond catalyzed by arylsulfatase from Helix Pomatia" 8th European Biological Chemistry Conference, EUROBIC 8, Aveiro, Portugalia, 2006
  20. K.Kurpiewska, K. Dziubek, A. Katrusiak, J. Font, M. Ribó, M. Vilanova, K. Lewiński "Crystallographic investigation of structural stability of RNase A at high pressure" 3rd Central European Conference Chemistry Towards Biology, Kraków, 8-13 września 2006
  21. I. Stawoska, S. Gawęda, P. Laidler, K. Lewiński, G. Stochel "Investigation into the mechanism of the biological activity of sulfatase from Helix pomatia" Inorganic Reaction Mechanisms Meeting, Kraków, 4-7.01.2006
  22. K. Lewiński, M. Bielak, P. Laidler "Enzymatic mechanism and inhibition of sulfatases as determined by X-ray crystallography" Zjazd Polskiego Towarzystwa Biochemicznego, Lublin, 19-23 wrzesień, 2005
  23. K. Lewiński, K. Kurpiewska, J. Font, M. Ribó, M. Vilanova "X-ray structural studies of ribonuclease A variants Trends in High Pressure Protein Sciences" Montpellier, Francja, 1-3 wrzesień, 2005
  24. K. Kurpiewska, J. Font, M. Ribó, M. Vilanova, K. Lewiński "X-ray structural characterization of RNase A variants engineered at the most destabilizing positions of the main hydrophobic core" International School of Crystallography, 37th Course “Evolving Methods For Macromolecular Crystallography”, Włochy, Erice 12-22 maja 2005r.
  25. K. Lewinski "Structural investigations of arylsulfatase A" Symposium Crystallography of Biomolecules, Łódź, 4 październik 2004
  26. K. Lewinski "Enzymatic mechanism of sulfatases" 2nd Conference Chemistry towards biology, Graz, 26-29 wrzesień 2004
  27. K. Lewinski, M. Chruszcz, P. Laidler, M. Bielak, M. Monkiewicz, E. Ortlund, L. Lebioda "Structural studies of covalent intermediate of human arylsulfatase" 21st European Crystallographic Meeting, Durban, RPA, 2003
  28. K. Lewinski "From Structure to Mechanism of Enzyme: the case of glutaminase-asparaginase" 1st Central European Conference Chemistry towards biology", Portoroz, Slowenia, 2002
  29. M. Chruszcz, P. Laidler, M. Monkiewicz, L. Lebioda, E. Ortlund, K. Lewinski "Inhibition of Human Arylsulfatase A" XLV Zjazd Naukowy PTChem. Sympozjum Sekcji Krystalochemii, Kraków-Mogilany, 2002
  30. D. R. Tomchick, K. Lewinski, A. Kiyatkin, T. Holman, J. Steczko, B. Axelrod, W. Minor "Lipoxygenase revisited" International Union of Crystallography XVIII Congress and General Assembly, Glasgow, Wielka Brytania, 1999
  31. K. Lewinski, C. G. Jakob, R. Kuciel, A. Mazurkiewicz, W. Ostrowski, L. Lebioda "The Crystal Structure of Human Prostatic Acid Phosphatase" XLI Konwersatorium Krystalograficzne, Wroclaw, 1999
  32. E. Ortlund, L. Lebioda, M. Chruszcz, D. Ksiazek, P. Laidler, K. Lewinski "Crystal structure of human placental arylsulfatase A - phosphotyrosine complex at 3 A resolution" 8-th Annual Conference on Molecular Approaches to Biological Problems, Isle of Palms, South Carolina, USA, 1999
  33. D. R. Tomchick, K. Lewinski, A. Kiyatkin, T. Holman, J. Steczko, B.Axelrod, W. Minor "Structural and mechanistic studies of soybean L-1 lipoxygenase" Annual Meeting of American Crystallographic Association, Arlington, USA, 1998



 

Modified by: Mariusz Pilch, 2014-11-05 09:53:12
   

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