| Metabolomics | |
| Unit Code | ASC 16 |
| Credits | 5 |
| Organic chemistry (basics) | Eurobachelor in chemistry or equivalent |
| TEACHING STAFF | Prof. W. Piekoszewski, mgr Agatha Grela |
COURSE DESCRIPTION: The course combines theoretical and practical aspects of metabolomics, which analyzes the low-weight molecules (metabolomes) that represent substrates, products and intermediate metabolites participating in biochemical pathways responsible for metabolism. Metabolomics can be defined as a discipline dedicated to the study of metabolites, their dynamics, composition, interactions and responses to interventions, changes in their environment, in cells, tissuses and biofluids. Metabolomics allows for determining disease markers, susceptibility to diseases, and even correct a patient’s diet to improve health condition. Due to metabolomes being low-weight molecules, their analysis requires using highly sensitive and accurate techniques, i.a. mass spectrometry.
AIMS:
- To provide understanding of metabolomics and present its applications in various scientific fields
- To extend the student’s practical knowledge of mass spectrometry
- To introduce the student to practical application of mass spectrometry in metabolomics
INTENDED LEARNING OUTCOMES:
After completing this unit the student should be able to:
- Present extensive knowledge of metabolomics and explain its importance
- Choose the most appropriate mass spectrometry technique and experimental setup for a given metabolomic problem
- Prepare and conduct an experiment applying gained knowledge in metabolomics
- Interpret gathered experimental data and present it in a written and/or oral form
TEACHING AND LEARNING ACTIVITIES:
Lectures and laboratory classes: 45 hours
Student centred learning: 75 hours
Total student effort: 120 hours
ASSESSMENT:
Examination on completion of teaching period: written or oral
RECOMMENDED READING:
- Orešič M., Metabolomics, a novel tool for studies of nutrition, metabolism and lipid dysfunction, Nutrition, Metabolism & Cardiovascular Diseases (2009) 19, 816-824
- Wolfram Weckwerth, Katja Morgenthal, Metabolomics: from pattern recognition to biological interpretation, DDT • Volume 10, Number 22 • November 2005
- B. Alvarez-Sanchez, F. Priego-Capote, M.D. Luque de Castro, Metabolomics analysis I. Selection of biological samples and practical aspects preceding sample preparation, Trends in Analytical Chemistry, Vol. 29, No. 2, 2010
- B. Alvarez-Sanchez, F. Priego-Capote, M.D. Luque de Castro, Metabolomics analysis II. Preparation of biological samples prior to detection, Trends in Analytical Chemistry, Vol. 29, No. 2, 2010
RECOMMENDED WEBSITES: